Skip to main content

IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo

  • Protocol
  • First Online:
Streptococcus pneumoniae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1968))

Abstract

Imaging through the IVIS Spectrum CT system does not provide the resolution at cellular level like the high-resolution or super-resolution microscopy. Rather, it detects bacterial infections in specific anatomical compartments/organs of the animals. The IVIS Spectrum imaging system is a unique imaging technology that allows for real-time monitoring of disease progression in living animals through the use of either bioluminescent or fluorescent probes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Poussard A, Patterson M, Taylor K et al (2012) In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus. J Vis Exp 70:e4429

    Google Scholar 

  2. Chen H, Thorne SH (2012) Practical methods for molecular in vivo optical imaging. Curr Protoc Cytom 59:12–24

    PubMed  PubMed Central  Google Scholar 

  3. Iovino F, Engelen-Lee JY, Brouwer M (2017) pIgR and PECAM-1 bind to pneumococcal RrgA and PspC mediating bacterial invasion of the brain. J Exp Med 214:1619–1630

    Article  CAS  Google Scholar 

  4. Iovino F, Thorsdottir S, Henriques-Normark B (2018) Receptor blockade: a novel approach to protect the brain from pneumococcal invasion. J Infect Dis 218(3):476–484. https://doi.org/10.1093/infdis/jiy193

    Article  PubMed  Google Scholar 

  5. Orihuela CJ, Gao G, Francis KP (2004) Tissue-specific contributions of pneumococcal virulence factors to pathogenesis. J Infect Dis 190:1661–1669

    Article  CAS  Google Scholar 

  6. Ravensdale J, Wong Z, O’Brien F (2016) Efficacy of antibacterial peptides against peptide resistant MRSA is restored by permeabilization of bacteria membranes. Front Microbiol 7:1745

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Federico Iovino .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Sierakowiak, A., Henriques-Normark, B., Iovino, F. (2019). IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo. In: Iovino, F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9199-0_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-9199-0_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9198-3

  • Online ISBN: 978-1-4939-9199-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics