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Generation of Skeletal Myocytes from Embryonic Stem Cells Through Nuclear Receptor Signaling

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Nuclear Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1966))

Abstract

The differentiation of mouse embryonic stem (ES) cells into skeletal myocytes can be enhanced by small molecular inducers, which signal through muscle-specific transcription networks. However, to induce differentiation in vitro, the ES cells must be thrusted through the stage of embryoid body (EB) formation. Here, we describe how to efficiently direct the commitment of ES cells into skeletal muscle lineage by using nuclear receptor agonists in a hanging drop protocol.

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Acknowledgments

This work was sponsored by operating grant from the Natural Sciences and Engineering Research Council of Canada.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Jihong Chen & Qiao Li

  2. Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Hongsheng Liang, Aili Gao & Qiao Li

Authors
  1. Jihong Chen
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  2. Hongsheng Liang
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  3. Aili Gao
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  4. Qiao Li
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Corresponding author

Correspondence to Qiao Li .

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Editors and Affiliations

  1. School of Pharmacy, University of Missouri-Kansas City, Kansas City, MO, USA

    Mostafa Z. Badr

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Chen, J., Liang, H., Gao, A., Li, Q. (2019). Generation of Skeletal Myocytes from Embryonic Stem Cells Through Nuclear Receptor Signaling. In: Badr, M. (eds) Nuclear Receptors. Methods in Molecular Biology, vol 1966. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9195-2_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9195-2_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9194-5

  • Online ISBN: 978-1-4939-9195-2

  • eBook Packages: Springer Protocols

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