Abstract
Steroid receptors are members of a subfamily of the nuclear receptor superfamily. They play a dual role of steroid hormone receptors and transcription factors. Actually, these receptors are steroid-activated transcription factors. Classical soluble receptors exist as oligomeric complexes with the Hsp90-based chaperone machinery. The steroid receptor field was born and developed along with the molecular chaperone field. Chaperones are not exclusive partners associated to these receptors, but also comprise a large variety of heterocomplexes with other proteins involved in signal transduction. By using the glucocorticoid receptor (GR) as a standard model for most Hsp90-client proteins, in this chapter we describe the functional GR·Hsp90 heterocomplex assembly system from reticulocyte lysate or purified proteins.
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Acknowledgments
The research work in our laboratory is supported by grants from the University of Buenos Aires (UBACYT Program) and Agencia Nacional de Promoción Científica y Tecnológica (PICT 2014-3433 and PICT 2016-0545 to M.D.G., and PICT 2016-2607 to G.I.M.).
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Mazaira, G.I., Galigniana, M.D. (2019). Reconstitution of the Steroid Receptor Heterocomplex. In: Badr, M. (eds) Nuclear Receptors. Methods in Molecular Biology, vol 1966. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9195-2_10
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DOI: https://doi.org/10.1007/978-1-4939-9195-2_10
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