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Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish

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Book cover Developmental Toxicology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1965))

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Abstract

Forward genetic screening is an extremely powerful method for identifying novel genes driving a broad range of phenotypes. This protocol describes the complete process for conducting a forward genetic screen in zebrafish, including mutagenesis with N-ethyl-N-nitrosourea (ENU), mating, phenotypic screening, and genetic mapping.

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References

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Author information

Authors and Affiliations

  1. Department of Physiology and Developmental Biology, College of Life Sciences, Brigham Young University, Provo, UT, USA

    Jonathon T. Hill

Authors
  1. Jonathon T. Hill
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Correspondence to Jonathon T. Hill .

Editor information

Editors and Affiliations

  1. Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA

    Jason M. Hansen

  2. Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Louise M. Winn

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Hill, J.T. (2019). Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish. In: Hansen, J., Winn, L. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 1965. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9182-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9182-2_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9181-5

  • Online ISBN: 978-1-4939-9182-2

  • eBook Packages: Springer Protocols

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