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Microcalorimetry of Biological Molecules pp 3–15Cite as

The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1964))

Abstract

Membrane-active peptides include a variety of molecules such as antimicrobial (AMP), cell-penetrating (CPP), viral, and amyloid peptides that are implicated in several pathologies. They constitute important targets because they are either at the basis of novel therapies (drug delivery for CPPs or antimicrobial activity for AMPs) or they are the agents causing these pathologies (viral and amyloid peptides). They all share the common property of interacting with the cellular lipid membrane in their mode of action. Therefore, a better understanding of the peptide/lipid (P/L) interaction is essential to help decipher their mechanism of action. Among the different biophysical methods that can be used to fully characterize P/L interactions, differential scanning calorimetry (DSC) allows determining the peptide effect on the lipid phase transitions, a property that reflects the P/L interaction mode. A general protocol for classical DSC experiments for P/L studies will be provided.

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Author information

Authors and Affiliations

  1. Institute for Pharmacology and Toxicology, Rudolf Virchow Center—Bio-Imaging Center, University of Würzburg, Würzburg, Germany

    Marie-Lise Jobin

  2. Chimie et Biologie des Membranes et Nanoobjets, CBMN CNRS UMR 5248, Université Bordeaux 1, Pessac, France

    Isabel D. Alves

Authors
  1. Marie-Lise Jobin
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  2. Isabel D. Alves
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Corresponding author

Correspondence to Isabel D. Alves .

Editor information

Editors and Affiliations

  1. Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, CNRS, Strasbourg, France

    Eric Ennifar

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Jobin, ML., Alves, I.D. (2019). The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions. In: Ennifar, E. (eds) Microcalorimetry of Biological Molecules. Methods in Molecular Biology, vol 1964. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9179-2_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9179-2_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9178-5

  • Online ISBN: 978-1-4939-9179-2

  • eBook Packages: Springer Protocols

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