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Ancient DNA pp 57–63Cite as

DNA Extraction from Keratin and Chitin

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1963))

Abstract

DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica column or organic methods.

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References

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Acknowledgment

M.T.P.G. was supported by the Danish National Science Foundation’s “Skou” grant program.

Author information

Authors and Affiliations

  1. Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark

    Paula F. Campos & M. Thomas P. Gilbert

Authors
  1. Paula F. Campos
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  2. M. Thomas P. Gilbert
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Editor information

Editors and Affiliations

  1. Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, USA

    Beth Shapiro

  2. Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany

    Axel Barlow

  3. Tromsø University Museum, UiT—The Arctic University of Norway, Tromsø, Norway

    Peter D. Heintzman

  4. Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany

    Michael Hofreiter

  5. Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany

    Johanna L. A. Paijmans

  6. Laboratório Nacional de Computação Científica, Petrópolis, Rio de Janeiro, Brazil

    André E. R. Soares

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Campos, P.F., Gilbert, M.T.P. (2019). DNA Extraction from Keratin and Chitin. In: Shapiro, B., Barlow, A., Heintzman, P., Hofreiter, M., Paijmans, J., Soares, A. (eds) Ancient DNA. Methods in Molecular Biology, vol 1963. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9176-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9176-1_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9175-4

  • Online ISBN: 978-1-4939-9176-1

  • eBook Packages: Springer Protocols

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