Abstract
Programmable nucleases like CRISPR/Cas9 enable to edit the mouse genome directly in the zygote. Several methods have been successfully used for this. Here we describe injection into one of the pronuclei of the zygote and electroporation of zygotes. Alternative methods will be mentioned.
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References
Oliveros JC, Franch M, Tabas-Madrid D, San-León D, Montoliu L, Cubas P, Pazos F (2016) Breaking-Cas-interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes. Nucleic Acids Res 44:W267–W271
Behringer R, Gertsenstein M, Nagy KV, Nagy A (2014) Manipulating the mouse embryo: a laboratory manual, 4th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Pease S, Saunders TL (2011) Advanced protocols for animal transgenesis, an ISTT manual. Springer, Berlin, Heidelberg
Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T, Redder R, Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL, Ohtsuka M, Gurumurthy CB (2017) Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol 18:92
Tröder SE, Ebert LK, Butt L, Assenmacher S, Schermer B, Zevnik B (2018) An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes. bioRxiv. 281402. https://doi.org/10.1101/281402
Wefers B, Bashir S, Rossius J, Wurst W, Kühn R (2017) Gene editing in mouse zygotes using the CRISPR/Cas9 system. Methods 121–122:55–67
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Hansen, L.A., Füchtbauer, EM. (2019). Genome Editing in Mice. In: Luo, Y. (eds) CRISPR Gene Editing. Methods in Molecular Biology, vol 1961. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9170-9_15
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DOI: https://doi.org/10.1007/978-1-4939-9170-9_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9169-3
Online ISBN: 978-1-4939-9170-9
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