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CRISPR-gRNA Design

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CRISPR Gene Editing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1961))

Abstract

Gene editing has great therapeutic impact, being of interest for many scientists worldwide. Clustered regularly interspaced short palindromic repeats (CRISPR) technology has been adapted for gene editing to serve as an efficient, rapid, and cost-effective tool. To fulfill CRISPR experiment’s goals, two components are important: an endonuclease and a gRNA. The most commonly used endonucleases are Cpf1 and Cas9 and are described in depth in this chapter. The gRNA targets the genome site to be edited, giving great importance to its design to obtain increased efficiency and decreased off-target events. In this chapter, we describe different tools to design suitable gRNAs for a variety of experimental purposes.

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Authors and Affiliations

  1. Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain

    Maria Pallarès Masmitjà, Nastassia Knödlseder & Marc Güell

Authors
  1. Maria Pallarès Masmitjà
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  2. Nastassia Knödlseder
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  3. Marc Güell
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Corresponding author

Correspondence to Marc Güell .

Editor information

Editors and Affiliations

  1. Department of Biomedicine, Aarhus University, Aarhus, Denmark

    Yonglun Luo

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Pallarès Masmitjà, M., Knödlseder, N., Güell, M. (2019). CRISPR-gRNA Design. In: Luo, Y. (eds) CRISPR Gene Editing. Methods in Molecular Biology, vol 1961. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9170-9_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9170-9_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9169-3

  • Online ISBN: 978-1-4939-9170-9

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