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Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils

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Book cover Mouse Models of Innate Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1960))

Abstract

Neutrophils are some of the first leukocytes to respond to inflammatory stimuli. Once recruited, these cells are equipped with an assortment of proteolytic enzymes and antimicrobial factors that disarm and degrade pathogens. Neutrophils employ a highly novel mechanism to contain and trap bacteria in the local inflammatory microenvironment, termed neutrophil extracellular traps (NETs). During NET formation, neutrophils eject weblike structures of chromatin, which captures and immobilizes invading pathogens. In this chapter, we describe protocols to isolate bone marrow-derived neutrophils from mice. We further describe in vitro methods to spectrophotometrically quantify, immunolabel, and visualize NET structures.

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Author information

Authors and Affiliations

  1. Cell Biology Program, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, ON, Canada

    Linda Vong & Philip M. Sherman

  2. Faculty of Dentistry, University of Toronto, Toronto, ON, Canada

    Philip M. Sherman & Michael Glogauer

Authors
  1. Linda Vong
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  2. Philip M. Sherman
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  3. Michael Glogauer
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Corresponding author

Correspondence to Michael Glogauer .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, Virginia-Maryland Regional College, Blacksburg, VA, USA

    Irving C. Allen

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Vong, L., Sherman, P.M., Glogauer, M. (2019). Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1960. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9167-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9167-9_5

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9166-2

  • Online ISBN: 978-1-4939-9167-9

  • eBook Packages: Springer Protocols

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