Abstract
Selected/multiple reaction monitoring-mass spectrometry (SRM/MRM) is an analytical method that is frequently combined to the use of stable isotope-labeled standard (SIS) peptides for absolute protein quantification. The application of SRM/MRM is a relatively recent development in the proteomics field for analysis of biological samples (plasma, urine, cell/tissue lysates) targeting to a large extent biomarker validation. Although MRM generally by-passes the use of antibodies (being linked to sub-optimal assay specificity in many cases), bioanalytical validation of MRM protocols has not been robustly appliedĀ because of sensitivity issues, in contrary to antibody-based methods. In this chapter, we will discuss the points that should be addressed for MRM method development in clinical proteomics applications.
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Support for this chapter was offered by the Greek GSRT (grant mELISA T1EĪK-03551)Ā and the EU COST action CliniMARK (CA16113).
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Kontostathi, G., Makridakis, M., Bitsika, V., Tsolakos, N., Vlahou, A., Zoidakis, J. (2019). Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications. In: Brun, V., CoutƩ, Y. (eds) Proteomics for Biomarker Discovery. Methods in Molecular Biology, vol 1959. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9164-8_14
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