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Beta-Arrestins pp 139–158Cite as

Methods to Characterize Protein Interactions with β-Arrestin In Cellulo

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1957))

Abstract

β-Arrestins 1 and 2 (β-arr1 and β-arr2) are ubiquitous proteins with common and distinct functions. They were initially identified as proteins recruited to stimulated G protein-coupled receptors (GPCRs), regulating their desensitization and internalization. The discovery that β-arrs could also interact with more than 400 non-GPCR protein partners brought to light their central roles as multifunctional scaffold proteins regulating multiple signalling pathways from the plasma membrane to the nucleus, downstream of GPCRs or independently from these receptors. Through the regulation of the activities and subcellular localization of their binding partners, β-arrs control various cell processes such as proliferation, cytoskeletal rearrangement, cell motility, and apoptosis. Thus, the identification of β-arrs binding partners and the characterization of their mode of interaction in cells are central to the understanding of their function. Here we provide methods to explore the molecular interaction of β-arrs with other proteins in cellulo.

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Authors and Affiliations

  1. Institut Cochin, INSERM U1016, CNRS UMR8104, Université Paris Descartes, Sorbonne Paris Cité, Paris, France

    Revu Ann Alexander, Isaure Lot & Hervé Enslen

Authors
  1. Revu Ann Alexander
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  2. Isaure Lot
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  3. Hervé Enslen
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Corresponding author

Correspondence to Hervé Enslen .

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Editors and Affiliations

  1. Institut Cochin, INSERM U1016, Paris, France

    Mark G. H. Scott

  2. Department of Medicine, Research Institute of the McGill, University Health Center (RI-MUHC), McGill University, Montreal, QC, Canada

    Stéphane A. Laporte

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Alexander, R.A., Lot, I., Enslen, H. (2019). Methods to Characterize Protein Interactions with β-Arrestin In Cellulo. In: Scott, M., Laporte, S. (eds) Beta-Arrestins. Methods in Molecular Biology, vol 1957. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9158-7_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9158-7_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9157-0

  • Online ISBN: 978-1-4939-9158-7

  • eBook Packages: Springer Protocols

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