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Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides

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Bacterial Polysaccharides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1954))

Abstract

The polysaccharides that comprise bacterial cell walls are commonly O-acetylated. This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity. Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases. The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.

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Acknowledgments

Our research on O-acetylesterases and O-acetyltransferases has been supported by operating grants from the Canadian Institutes for Health Research, and more recently, the Canadian Glycomics Network, a National Centre of Excellence.

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada

    Ashley S. Brott, David Sychantha & Anthony J. Clarke

Authors
  1. Ashley S. Brott
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  2. David Sychantha
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  3. Anthony J. Clarke
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Corresponding author

Correspondence to Anthony J. Clarke .

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Editors and Affiliations

  1. Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Inka Brockhausen

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Brott, A.S., Sychantha, D., Clarke, A.J. (2019). Assays for the Enzymes Catalyzing the O-Acetylation of Bacterial Cell Wall Polysaccharides. In: Brockhausen, I. (eds) Bacterial Polysaccharides. Methods in Molecular Biology, vol 1954. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9154-9_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9154-9_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9153-2

  • Online ISBN: 978-1-4939-9154-9

  • eBook Packages: Springer Protocols

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