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Lymphoma pp 249–267Cite as

FISH and FICTION in Lymphoma Research

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1956))

Abstract

Fluorescence in situ hybridization (FISH) is a powerful and robust technique allowing the visualization of target sequences like genes in interphase nuclei. It is widely used in routine diagnostics to identify cancer-specific aberrations including lymphoma-associated translocations or gene copy number changes in single tumor cells. By combining FISH with immunophenotyping—a technique called fluorescence immunophenotyping and interphase cytogenetic as a tool for investigation of neoplasia (FICTION)—it is moreover possible to identify a cell population of interest. Here we describe standard protocols for FISH and FICTION as used in our laboratories in diagnosis and research.

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Acknowledgments

The work of the authors on FISH/FICTION has been supported by the Polish Ministry of Science and Higher Education (grant IP2012 012072) to M.G. and from the Deutsche Krebshilfe, the Kinderkrebsinitiative Buchholz/Holm-Seppensen (KKI), and the BMBF to R.S.

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Authors and Affiliations

  1. Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

    Maciej Giefing

  2. Institute of Human Genetics, Ulm University and Ulm University Medical Center, Ulm, Germany

    Reiner Siebert

Authors
  1. Maciej Giefing
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  2. Reiner Siebert
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Corresponding author

Correspondence to Maciej Giefing .

Editor information

Editors and Affiliations

  1. Institute of Cell Biology (Cancer Research), Medical School, University of Duisburg-Essen, Nordrhein-Westfalen, Essen, Germany

    Ralf Küppers

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Giefing, M., Siebert, R. (2019). FISH and FICTION in Lymphoma Research. In: Küppers, R. (eds) Lymphoma. Methods in Molecular Biology, vol 1956. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9151-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9151-8_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9150-1

  • Online ISBN: 978-1-4939-9151-8

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