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T. cruzi Infection pp 135–146Cite as

Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1955))

Abstract

Trypanosoma cruzi, the protozoan agent of Chagas disease, has evolved an innovative metabolic pathway by which protective sialic acid (SA) residues are scavenged from host sialylglycoconjugates and transferred onto parasite surface mucin-like molecules (or surface glycoconjugates from host target cells) by means of a unique trans-sialidase (TS) enzyme. TS-induced changes in the glycoprotein sialylation profile of both parasite and host cells are crucial for the establishment of a persistent T. cruzi infection and for the development of Chagas disease-associated pathogenesis. In this chapter, we describe a novel metabolic labeling method developed in our labs that enables straightforward identification and molecular characterization of SA acceptors of the TS-catalyzed reaction.

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Acknowledgments

GC and MS hold fellowships from CONICET, and MdlMC, CAB, OC and JM are career investigators from the same Institution. This work was supported by the National Institute of Health R01AI104531 to OC, and Agencia Nacional de Promoción Científica y Tecnológica PICT2012-1815 to JM. The funders had no role in decision to publish or preparation of the manuscript.

Author information

Author notes

  1. Andrés B. Lantos

    Present address: Laboratorio Dr. Lantos, Buenos Aires, Argentina

  2. Gaspar E. Cánepa

    Present address: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA

Authors and Affiliations

  1. Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina

    Giannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia, Oscar Campetella & Juan Mucci

  2. Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina

    Giannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia, Oscar Campetella & Juan Mucci

  3. Instituto de Tecnología, Universidad Argentina de la Empresa (UADE), Buenos Aires, Argentina

    María de los Milagros Cámara

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  1. Giannina Carlevaro
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  2. Andrés B. Lantos
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  3. Gaspar E. Cánepa
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  4. María de los Milagros Cámara
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  5. Martín Somoza
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  6. Carlos A. Buscaglia
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  7. Oscar Campetella
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  8. Juan Mucci
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Correspondence to Juan Mucci .

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Editors and Affiliations

  1. INGEBI-CONICET, Buenos Aires, Argentina

    Karina Andrea Gómez

  2. UNSAM-CONICET, IIB-INTECH, San Martin, Buenos Aires, Argentina

    Carlos Andrés Buscaglia

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Carlevaro, G. et al. (2019). Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase. In: Gómez, K., Buscaglia, C. (eds) T. cruzi Infection. Methods in Molecular Biology, vol 1955. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9148-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9148-8_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9147-1

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