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Using Functional Genetics in Haploid Cells for Drug Target Identification

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Book cover Target Identification and Validation in Drug Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1953))

Abstract

Pooled genetic screens are a powerful tool to identify targets for drug development as well as chemogenetic interactions. Various complementary methods for mutagenesis are available to generate highly complex cell populations, including mRNA knockdown, directed genome editing, as well as random genome mutagenesis. With the availability of a growing number of haploid mammalian cell lines, random mutagenesis is becoming increasingly powerful and represents an attractive alternative, e.g., to CRISPR-based screening. This chapter provides a step-by-step protocol for performing haploid gene trap screens.

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Authors and Affiliations

  1. IMBA - Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria

    Jennifer C. Volz, Nicole Schuller & Ulrich Elling

Authors
  1. Jennifer C. Volz
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  2. Nicole Schuller
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  3. Ulrich Elling
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Corresponding author

Correspondence to Ulrich Elling .

Editor information

Editors and Affiliations

  1. Pharmacology and Translational Research, Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria

    Jürgen Moll

  2. Department of Cancer Cell Signaling, Boehringer Ingelheim RCV GmBH & Co KG, Vienna, Austria

    Sebastian Carotta

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Volz, J.C., Schuller, N., Elling, U. (2019). Using Functional Genetics in Haploid Cells for Drug Target Identification. In: Moll, J., Carotta, S. (eds) Target Identification and Validation in Drug Discovery. Methods in Molecular Biology, vol 1953. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9145-7_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9145-7_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9144-0

  • Online ISBN: 978-1-4939-9145-7

  • eBook Packages: Springer Protocols

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