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Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System

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Adeno-Associated Virus Vectors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1950))

Abstract

Intranasal delivery of solutions is a straightforward methodology for viral vector transduction and gene transfer to the epithelia within the nasal cavity. Beyond the simplicity of the technique, intranasal delivery has demonstrated restricted transduction of the olfactory and respiratory epithelial tissues. Here we outline the procedure of viral vector intranasal delivery in early postnatal and adult mice, as well as adult rats. The procedure allows for robust transduction and ectopic gene delivery that can be used for the visualization of cellular structures, protein distribution, and assessment of viral vector-mediated therapies.

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Acknowledgments

Intellectual support was provided by members of the University of Florida Center for Smell and Taste. This work was supported by National Institutes of Health R01DC009606 (J.R.M.).

Competing Interests

The authors declare no competing or financial interests.

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA

    Cedric R. Uytingco & Jeffrey R. Martens

  2. Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL, USA

    Cedric R. Uytingco & Jeffrey R. Martens

Authors
  1. Cedric R. Uytingco
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  2. Jeffrey R. Martens
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Corresponding author

Correspondence to Jeffrey R. Martens .

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Editors and Affiliations

  1. Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA

    Michael J. Castle

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Uytingco, C.R., Martens, J.R. (2019). Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System. In: Castle, M. (eds) Adeno-Associated Virus Vectors. Methods in Molecular Biology, vol 1950. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9139-6_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9139-6_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9138-9

  • Online ISBN: 978-1-4939-9139-6

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