Abstract
Adeno-associated Virus (AAV) vectors are useful vehicles for delivering transgenes to a number of different tissues and organs in vivo. To date, most of these applications deliver the vectors to their target by either infusion into the bloodstream or direct injection into the target tissue. Recently there has been progress in delivering AAV vectors to neurons of the peripheral nervous system (PNS) following application of vectors to the peripheral epithelium, such as the skin or eye. This delivery only requires treatment of the epithelium to access the underlying nerve termini, and following treatment the vectors are transported retrogradely to the cell bodies of these neurons in the ganglia, such as dorsal root ganglia (DRG) or trigeminal ganglia (TG). Here we describe the methodology for highly efficient transduction of mouse DRG and rabbit TG following application of AAV vectors to the foot, or to the cornea, respectively.
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References
Moore NA, Morral N, Ciulla TA, Bracha P (2018) Gene therapy for inherited retinal and optic nerve degenerations. Expert Opin Biol Ther 18(1):37–49. https://doi.org/10.1080/14712598.2018.1389886
Blessing D, Deglon N (2016) Adeno-associated virus and lentivirus vectors: a refined toolkit for the central nervous system. Curr Opin Virol 21:61–66. https://doi.org/10.1016/j.coviro.2016.08.004
Watson ZL, Ertel MK, Lewin AS, Tuli SS, Schultz GS, Neumann DM, Bloom DC (2016) Adeno-associated virus vectors efficiently transduce mouse and rabbit sensory neurons coinfected with herpes simplex virus 1 following peripheral inoculation. J Virol 90(17):7894–7901. https://doi.org/10.1128/JVI.01028-16
Aubert M, Madden EA, Loprieno M, DeSilva Feelixge HS, Stensland L, Huang ML, Greninger AL, Roychoudhury P, Niyonzima N, Nguyen T, Magaret A, Galleto R, Stone D, Jerome KR (2016) In vivo disruption of latent HSV by designer endonuclease therapy. JCI Insight 1(14). https://doi.org/10.1172/jci.insight.88468
Dang CH, Aubert M, De Silva Feelixge HS, Diem K, Loprieno MA, Roychoudhury P, Stone D, Jerome KR (2017) In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia. Sci Rep 7(1):927. https://doi.org/10.1038/s41598-017-01004-y
Watson ZL, Washington SD, Phelan DM, Lewin AS, Tuli SS, Schultz GS, Neumann DM, Bloom DC (2018) In vivo knockdown of the herpes simplex virus 1 latency-associated transcript reduces reactivation from latency. J Virol 92(16). https://doi.org/10.1128/JVI.00812-18
Washington SD, Edenfield SI, Lieux C, Watson ZL, Taasan SM, Dhummakupt A, Bloom DC, Neumann DM (2018) Depletion of the insulator protein CTCF results in HSV-1 reactivation in vivo. J Virol. https://doi.org/10.1128/JVI.00173-18
Zolotukhin S, Potter M, Zolotukhin I, Sakai Y, Loiler S, Fraites TJ Jr, Chiodo VA, Phillipsberg T, Muzyczka N, Hauswirth WW, Flotte TR, Byrne BJ, Snyder RO (2002) Production and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors. Methods 28(2):158–167. https://doi.org/10.1016/S1046-2023(02)00220-7
Zeier Z, Kumar A, Bodhinathan K, Feller JA, Foster TC, Bloom DC (2009) Fragile X mental retardation protein replacement restores hippocampal synaptic function in a mouse model of fragile X syndrome. Gene Ther 16(9):1122–1129. https://doi.org/10.1038/gt.2009.83
Bloom DC (1996) HSV vectors for gene therapy. In: Brown SM, MacLean AR (eds) Herpes simplex virus protocols, Methods in molecular medicine, vol 10. Humana Press, Totowa, pp 369–386
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Bloom, D.C., Watson, Z.L., Neumann, D.M. (2019). Peripheral AAV Injection for Retrograde Transduction of Dorsal Root and Trigeminal Ganglia. In: Castle, M. (eds) Adeno-Associated Virus Vectors. Methods in Molecular Biology, vol 1950. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9139-6_13
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DOI: https://doi.org/10.1007/978-1-4939-9139-6_13
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