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Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Production

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Intracellular Lipid Transport

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1949))

Abstract

Here we summarize how glycosphingolipid production can be followed using metabolic labeling with radiolabeled lipid precursors. No assays are available yet that directly would address the lipid transfer protein activity in vivo. Therefore, these approaches can serve as tools to indirectly study the lipid transfer protein activity in cells, by monitoring their impact on the glycosphingolipid homeostasis.

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Acknowledgments

This work was supported by Svenska Kulturfonden, Sigrid Jusélius Foundation, Magnus Ehrnrooth Foundation, Medicinska Understödsföreningen Liv och Hälsa, Svensk Österbottniska Samfundet.

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Authors and Affiliations

  1. Faculty of Science and Engineering, Biochemistry, Åbo Akademi University, Turku, Finland

    Anders P. E. Backman, Josefin Halin, Matti A. Kjellberg & Peter Mattjus

Authors
  1. Anders P. E. Backman
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  2. Josefin Halin
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  3. Matti A. Kjellberg
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  4. Peter Mattjus
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Corresponding author

Correspondence to Peter Mattjus .

Editor information

Editors and Affiliations

  1. Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur and CNRS, Valbonne, France

    Guillaume Drin

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Backman, A.P.E., Halin, J., Kjellberg, M.A., Mattjus, P. (2019). Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Production. In: Drin, G. (eds) Intracellular Lipid Transport. Methods in Molecular Biology, vol 1949. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9136-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9136-5_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9135-8

  • Online ISBN: 978-1-4939-9136-5

  • eBook Packages: Springer Protocols

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