Abstract
Matrigel is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma in C57BL/6 mice, a tumor rich in extracellular matrix (ECM) proteins. It consists mainly of laminin (approximately 60%), collagen IV (approximately 30%), and nidogen-1/entactin (approximately 8%), while it also contains heparan sulfate proteoglycans, such as perlecan, other ECM proteins, as well as growth factors bound to the ECM. Matrigel mimics the physiological cell matrix and is the most commonly used matrix substrate to study in vitro and in vivo angiogenesis. Here, we describe the in vivo Matrigel plug assay and how it can be used for both qualitative and quantitative assessment of angiogenesis.
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Acknowledgments
Pinelopi Kastana, Despoina Ntenekou and Stamatiki Katraki-Pavlou are recipients of a PhD scholarship from the State Scholarship Foundation in Greece (IKY) (Operational Program “Human Resources Development – Education and Lifelong Learning,” Partnership Agreement PA 2014-2020). This research was supported in part by a Marie Curie Intra European Fellowship within the 7th European Community Framework Programme (ALTangioTARGET) and by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases. All mice were bred and maintained under pathogen-free conditions at the University of Patras—Greece Animal Facility (EL13BIO04) or at American Association for the Accreditation of Laboratory Animal Care-accredited animal facilities at the NIAID or the TTUHSC School of Pharmacy and housed according to Directive 2010/63/EU (http://eur-lex.europa.eu/legal-content/EN/TXT/?uri=celex%3A32010L0063) or in accordance with the procedures outlined in the Guide for the Care and Use of Laboratory Animals under animal study proposals approved by the Veterinary Administration of Western Greece (approval # 118016/577/30-04-2014) or the NIAID or TTUHSC Animal Care and Use Committees, respectively. All mice were used between 6 and 13 weeks of age.
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Kastana, P. et al. (2019). Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis. In: Vigetti, D., Theocharis, A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9133-4_18
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DOI: https://doi.org/10.1007/978-1-4939-9133-4_18
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