Abstract
Divalent cations can change the actual electrical potential at the outer surface of the plasma membrane. They do so by the so-called Gouy-Chapman-Stern effect which is due to the electrical “masking” that certain ions, especially divalents, can exert onto the electrically negative charged polar heads of the membrane phospholipids.
Chondroitin sulfates can chelate free calcium ions to a different extent based on the spatial arrangement of their sulfate groups and can thus alter the actual availability of screening divalent ions at the outer membrane surface.
Voltage-dependent ion channels sense the actual potential difference between the two sides of the plasma membrane and are thus exquisite and extremely sensitive “devices” able to react to changes in the electrical potential across the membrane.
Hence, by recording the shift in the activation curve of well-known voltage-dependent ionic channels it will be possible to study the physical effect of ECM chondroitin sulfates on membrane conductances.
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References
Vigetti D, Andrini O, Clerici M et al (2008) Chondroitin sulfates act as extracellular gating modifiers on voltage-dependent ion channels. Cell Physiol Biochem 22:137–146
Piccolino M, Vellani V, Rakotobe LA et al (1999) Manipulation of synaptic sign and strength with divalent cations in the vertebrate retina: pushing the limits of tonic, chemical neurotransmission. Eur J Neurosci 11:4134–4138
Grahame DC (1947) The electrical double layer and the theory of electrocapillarity. Chem Rev 41:441–501
Frankenhaeuser B, Hodgkin AL (1957) The action of calcium on the electrical properties of squid axons. J Physiol 137:218–244
Hille B, Woodhull AM, Shapiro BI (1975) Negative surface charge near sodium channels of nerve: divalent ions, monovalent ions, and pH. Philos Trans R Soc Lond Ser B Biol Sci 270:301–318
Iozzo RV (1998) Matrix proteoglycans: from molecular design to cellular function. Annu Rev Biochem 67:609–652
Hunter GK, Wong KS, Kim JJ (1988) Binding of calcium to glycosaminoglycans: an equilibrium dialysis study. Arch Biochem Biophys 260:161–167
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Solari, E., Moriondo, A. (2019). Method for Studying the Physical Effect of Extracellular Matrix on Voltage-Dependent Ion Channel Gating. In: Vigetti, D., Theocharis, A.D. (eds) The Extracellular Matrix. Methods in Molecular Biology, vol 1952. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9133-4_13
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DOI: https://doi.org/10.1007/978-1-4939-9133-4_13
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