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Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy

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Alpha-Synuclein

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1948))

Abstract

Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is ideally suited to study structure, dynamics, and interactions of intrinsically disordered proteins as alpha-synuclein.

Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.

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References

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Acknowledgments

This work was supported by the DFG within SFB 969, project C03.

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Authors and Affiliations

  1. Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany

    Julia Cattani, Theresa Braun & Malte Drescher

Authors
  1. Julia Cattani
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  2. Theresa Braun
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  3. Malte Drescher
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Corresponding author

Correspondence to Malte Drescher .

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Editors and Affiliations

  1. Dementia Research Institute, University College London, London, UK

    Tim Bartels

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Cattani, J., Braun, T., Drescher, M. (2019). Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy. In: Bartels, T. (eds) Alpha-Synuclein. Methods in Molecular Biology, vol 1948. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9124-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9124-2_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9123-5

  • Online ISBN: 978-1-4939-9124-2

  • eBook Packages: Springer Protocols

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