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Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays

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G Protein-Coupled Receptor Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1947))

Abstract

Given the increasing amount of data showing the importance of protein tyrosine phosphatases (PTPs) in G protein-coupled receptor (GPCR) signaling pathways, the modulation of this enzyme family by that type of receptor can become an important experimental question. Here, we describe two different methods, an in-gel and a colorimetric PTP assay, to evaluate the modulation of PTP activity after stimulation with GPCR agonists.

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Acknowledgments

This work was supported by Canadian Institutes of Health Research grant (#MT6822).

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Authors and Affiliations

  1. Division of Immunology, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, QC, Canada

    Geneviève Hamel-Côté, Fanny Lapointe & Jana Stankova

Authors
  1. Geneviève Hamel-Côté
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  2. Fanny Lapointe
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  3. Jana Stankova
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Corresponding author

Correspondence to Jana Stankova .

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Editors and Affiliations

  1. Ottawa Hospital Research Institute (Neurosciences), Ottawa, ON, Canada

    Mario Tiberi

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Hamel-Côté, G., Lapointe, F., Stankova, J. (2019). Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays. In: Tiberi, M. (eds) G Protein-Coupled Receptor Signaling. Methods in Molecular Biology, vol 1947. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9121-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9121-1_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9120-4

  • Online ISBN: 978-1-4939-9121-1

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