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Acinetobacter baumannii pp 87–94Cite as

Vesicle-Mediated Gene Transfer in Acinetobacter baumannii

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

The role of vesicle-mediated gene transfer in Acinetobacter baumannii populations has been investigated in the last decade. Importantly, outer membrane vesicles (OMVs) secreted from A. baumannii cells have proven to be efficient agents of transfer of antimicrobial resistance genes to other bacterial species. However, the measurement of vesicle-mediated transfer depends on many experimental parameters. Here, we describe an experimental method useful to study transfer of DNA via membrane vesicles of A. baumannii in various bacterial populations.

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Author information

Authors and Affiliations

  1. Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, India

    Shweta Fulsundar

  2. Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal

    Sara Domingues

  3. Department of Life Sciences and Health, OsloMet—Oslo Metropolitan University, Oslo, Norway

    Kaare Magne Nielsen

Authors
  1. Shweta Fulsundar
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  2. Sara Domingues
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  3. Kaare Magne Nielsen
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Corresponding author

Correspondence to Kaare Magne Nielsen .

Editor information

Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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Fulsundar, S., Domingues, S., Nielsen, K.M. (2019). Vesicle-Mediated Gene Transfer in Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

  • eBook Packages: Springer Protocols

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