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Global Metabolic Analyses of Acinetobacter baumannii

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Acinetobacter baumannii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

Acinetobacter baumannii is rapidly emerging as a multidrug-resistant pathogen responsible for nosocomial infections including pneumonia, bacteremia, wound infections, urinary tract infections, and meningitis. Metabolomics provides a powerful tool to gain a system-wide snapshot of cellular biochemical networks under defined conditions and has been increasingly applied to bacterial physiology and drug discovery. Here we describe an optimized sample preparation method for untargeted metabolomics studies in A. baumannii. Our method provides a significant recovery of intracellular metabolites to demonstrate substantial differences in global metabolic profiles among A. baumannii strains.

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Acknowledgments

Jian Li, Darren J. Creek, and Tony Velkov are supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (R01 AI111965 and AI132681 [JL and TV]). Mohd Hafidz Mahamad Maifiah is an academic staff from the International Institute for Halal Research and Training (INHART), International Islamic University Malaysia (IIUM). Darren J. Creek and Tony Velkov are Australian National Health and Medical Research Council (NHMRC) Dareer Development Fellows. Jian Li is an Australian NHMRC Senior Research Fellow.

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Authors and Affiliations

  1. International Institute for Halal Research and Training, International Islamic University Malaysia, Gombak, Selangor, Malaysia

    Mohd Hafidz Mahamad Maifiah

  2. Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia

    Mohd Hafidz Mahamad Maifiah & Darren J. Creek

  3. Department of Pharmacology and Therapeutics, The University of Melbourne, Parkville, VIC, Australia

    Tony Velkov

  4. Laboratory of Antimicrobial Systems Pharmacology, Department of Microbiology, Biomedicine Discovery Institute, Monash University, Melbourne, VIC, Australia

    Jian Li

Authors
  1. Mohd Hafidz Mahamad Maifiah
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  2. Tony Velkov
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  3. Darren J. Creek
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  4. Jian Li
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Corresponding author

Correspondence to Jian Li .

Editor information

Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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Mahamad Maifiah, M.H., Velkov, T., Creek, D.J., Li, J. (2019). Global Metabolic Analyses of Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_28

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_28

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

  • eBook Packages: Springer Protocols

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