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Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection

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Acinetobacter baumannii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

Acinetobacter baumannii is a Gram-negative opportunistic pathogen and a leading cause of ventilator-associated pneumonia. Murine models of A. baumannii lung infection allow researchers to experimentally assess A. baumannii virulence and host response. Intranasal administration of A. baumannii models acute lung infection. This chapter describes the methods to test A. baumannii virulence in a murine model of lung infection, including assessing the competitive index of a bacterial mutant and the associated inflammatory responses.

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Acknowledgments

We thank Michael J. Noto, Zachery R. Lonergan, and Lillian J. Juttukonda for critical reading of the manuscript. This work was supported by the National Institutes of Health (NIH) research grant AI101171 to E.P.S. and fellowships F32AI122516 to L.D.P. and T32HL094296 to L.D.P. and E.R.G.

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Authors and Affiliations

  1. Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA

    Lauren D. Palmer, Erin R. Green, Jessica R. Sheldon & Eric P. Skaar

  2. Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA

    Lauren D. Palmer, Erin R. Green, Jessica R. Sheldon & Eric P. Skaar

Authors
  1. Lauren D. Palmer
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  2. Erin R. Green
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  3. Jessica R. Sheldon
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  4. Eric P. Skaar
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Corresponding author

Correspondence to Eric P. Skaar .

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Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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Palmer, L.D., Green, E.R., Sheldon, J.R., Skaar, E.P. (2019). Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_26

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

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