Abstract
Acinetobacter baumannii cell-cell signaling is mediated by production and release of N-acyl homoserine lactones (AHLs), primarily N-(3-hydroxydodecanoyl)-l-homoserine lactone (3-hydroxy-C12-HSL). The secretion of this signal can be readily assessed via simple plate-based or thin-layer chromatography-based assays utilizing an Agrobacterium tumefaciens traG-lacZ containing biosensor. In this chapter, we describe methods to assay for secreted N-acyl homoserine lactone production in Acinetobacter baumannii.
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References
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Acknowledgment
This work is supported by the following awards to P.N.R., VA Merit Award I01 BX001725 and Research Career Scientist Award IK6BX004470, both from the Department of Veterans Affairs, and R01AI072219 from the National Institutes of Health.
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Paulk Tierney, A.R., Rather, P.N. (2019). Methods for Detecting N-Acyl Homoserine Lactone Production in Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_23
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DOI: https://doi.org/10.1007/978-1-4939-9118-1_23
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