Abstract
Methods to determine the presence of a bacterial capsule typically involve the use of stains specific for capsule, the exclusion of a dye by the capsule or by electron microscopy. However, these procedures require equipment that may not be readily available to all researchers. Here we describe a method for extraction, visualization, and quantification of extracellular capsular polysaccharide from Acinetobacter baumannii with commonly used reagents and equipment.
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Acknowledgment
This work is supported by the following awards to P.N.R., VA Merit Award I01 BX001725 and Research Career Scientist Award IK6BX004470, both from the Department of Veterans Affairs, and R01AI072219 from the National Institutes of Health.
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Tipton, K.A., Rather, P.N. (2019). Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_21
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DOI: https://doi.org/10.1007/978-1-4939-9118-1_21
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