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Tn7-Based Single-Copy Insertion Vectors for Acinetobacter baumannii

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Acinetobacter baumannii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

Acinetobacter baumannii is considered a problematic Gram-negative pathogen due to its widespread resistance to antibiotics. Understanding of resistance mechanisms in A. baumannii is critical for designing new and effective therapeutic options. However, this is hampered by the lack of tools to carry out genetic manipulations in A. baumannii. Here, we describe methods to use a chromosomal mini-Tn7-based single-copy gene expression system in A. baumannii. This system can be effectively used for performing genetic complementation studies, for tagging with fluorescent proteins, or for reporter fusion assays.

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Acknowledgments

This work was supported by grants from the Natural Sciences and Engineering Council of Canada (NSERC, 2015-05550) and the Canadian Institutes of Health Research (CIHR, 201610PJT-376486) to AK. Original work in the HPS laboratory was funded in part by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Number U54 AI065357. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Microbiology, University of Manitoba, Winnipeg, MB, Canada

    Kaleigh Ducas-Mowchun, P. Malaka De Silva, Rakesh Patidar & Ayush Kumar

  2. Department of Molecular Genetics and Microbiology, College of Medicine, Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA

    Herbert P. Schweizer

  3. Manitoba Chemosensory Biology Group, University of Manitoba, Winnipeg, MB, Canada

    Ayush Kumar

Authors
  1. Kaleigh Ducas-Mowchun
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  2. P. Malaka De Silva
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  3. Rakesh Patidar
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  4. Herbert P. Schweizer
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  5. Ayush Kumar
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Corresponding author

Correspondence to Ayush Kumar .

Editor information

Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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Ducas-Mowchun, K., De Silva, P.M., Patidar, R., Schweizer, H.P., Kumar, A. (2019). Tn7-Based Single-Copy Insertion Vectors for Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

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