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A Biomimetic Model for Mineralization of Type-I Collagen Fibrils

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Collagen

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1944))

Abstract

The bone and dentin mainly consist of type-I collagen fibrils mineralized by hydroxyapatite (HAP) nanocrystals. In vitro biomimetic models based on self-assembled collagen fibrils have been widely used in studying the mineralization mechanism of type-I collagen. In this chapter, the protocol we used to build a biomimetic model for the mechanistic study of type-I collagen mineralization is described. Type-I collagen extracted from rat tail tendon or horse tendon is self-assembled into fibrils and mineralized by HAP in vitro. The mineralization process is monitored by cryoTEM in combination with two-dimensional (2D) and three-dimensional (3D) stochastic optical reconstruction microscopy (STORM), which enables in situ and high-resolution visualization of the process.

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Author information

Author notes

  1. Shasha Yao and Yifei Xu contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, Center for Biomaterials and Biopathways, Zhejiang University, Hangzhou, Zhejiang, China

    Shasha Yao, Changyu Shao & Ruikang Tang

  2. Laboratory of Materials and Interface Chemistry, Department of Chemical Engineering and Chemistry, Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Eindhoven, The Netherlands

    Yifei Xu & Nico A. J. M. Sommerdijk

  3. Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands

    Yifei Xu & Nico A. J. M. Sommerdijk

  4. School of Chemistry, University of Edinburgh, Edinburgh, UK

    Fabio Nudelman

Authors
  1. Shasha Yao
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  2. Yifei Xu
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  3. Changyu Shao
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  4. Fabio Nudelman
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  5. Nico A. J. M. Sommerdijk
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  6. Ruikang Tang
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Corresponding author

Correspondence to Ruikang Tang .

Editor information

Editors and Affiliations

  1. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    Irit Sagi

  2. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    Nikolaos A. Afratis

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Yao, S., Xu, Y., Shao, C., Nudelman, F., Sommerdijk, N.A.J.M., Tang, R. (2019). A Biomimetic Model for Mineralization of Type-I Collagen Fibrils. In: Sagi, I., Afratis, N. (eds) Collagen. Methods in Molecular Biology, vol 1944. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9095-5_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9095-5_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9094-8

  • Online ISBN: 978-1-4939-9095-5

  • eBook Packages: Springer Protocols

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