Abstract
Collagen is the most widely used substratum in cell culture and biomaterials applications. In this chapter, we describe a simple procedure to isolate collagen, which can be employed to a wide range of tissue sources, and subsequently use it to study the collagen crosslinking and stabilization abilities of various compounds. The protocol is designed for a multi-well format assay and thus can be used for simultaneous assessment of multiple number of compounds and can be easily adapted to a high-throughput screening setup.
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Krishnaswamy, V.R. (2019). Multi-well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen. In: Sagi, I., Afratis, N. (eds) Collagen. Methods in Molecular Biology, vol 1944. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9095-5_2
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DOI: https://doi.org/10.1007/978-1-4939-9095-5_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9094-8
Online ISBN: 978-1-4939-9095-5
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