Abstract
Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected has so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein (EGFP) expressing mice for the differentiation of transfected lung cell populations based on their antigen expression.
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Acknowledgments
This work was supported by “Meditrans,” an Integrated Project funded by the European Commission under the Sixth Framework (NMP4-CT-2006-026668). The authors are grateful to Tanja Dicke and Matthias Schiller for expert support.
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Merkel, O.M., Marsh, L.M., Garn, H., Kissel, T. (2019). Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery. In: Ogris, M., Sami, H. (eds) Nanotechnology for Nucleic Acid Delivery. Methods in Molecular Biology, vol 1943. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9092-4_24
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DOI: https://doi.org/10.1007/978-1-4939-9092-4_24
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