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Nanotechnology for Nucleic Acid Delivery pp 161–176Cite as

Layer-By-Layer Film Engineering for Sequential Gene Delivery

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1943))

Abstract

Layer-by-layer (LbL) films are assembled with poly(amido amine)s (PAAs), a type of polycations containing bioreducible disulfide bond, and DNA plasmids to enable LbL film degradation in physiologic conditions by reacting with glutathione or redox-active membrane proteins. The interior layer structure of the LbL films during assembly and disassembly is studied by atomic force microscopy (AFM), ellipsometry, dynamic light scattering (DLS), and fluorescence spectroscopy. Insertion of barrier layers in bioreducible LbL films is necessary to stabilize the interior layer structure and slow down the film degradation rate to achieve sequential gene delivery. Localized gene delivery from the LbL films is demonstrated using human embryonic kidney 293 (HEK 293) cells.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, Wayne State University, Detroit, MI, USA

    Lingxiao Xie, Yi Zou, Sean Carroll & Guangzhao Mao

  2. Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA

    Sean Carroll & Maria Muniz

Authors
  1. Lingxiao Xie
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  2. Yi Zou
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  3. Sean Carroll
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  4. Maria Muniz
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  5. Guangzhao Mao
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Corresponding author

Correspondence to Guangzhao Mao .

Editor information

Editors and Affiliations

  1. Laboratory of MacroMolecular Cancer Therapeutics (MMCT), Department of Pharmaceutical Chemistry, Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria

    Manfred Ogris

  2. Laboratory of MacroMolecular Cancer Therapeutics (MMCT), Department of Pharmaceutical Chemistry, Center of Pharmaceutical Sciences, Faculty of Life Sciences, University of Vienna, Vienna, Austria

    Haider Sami

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Xie, L., Zou, Y., Carroll, S., Muniz, M., Mao, G. (2019). Layer-By-Layer Film Engineering for Sequential Gene Delivery. In: Ogris, M., Sami, H. (eds) Nanotechnology for Nucleic Acid Delivery. Methods in Molecular Biology, vol 1943. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9092-4_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9092-4_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9091-7

  • Online ISBN: 978-1-4939-9092-4

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