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Mouse Hematopoietic Stem Cell Modification and Labelling by Transduction and Tracking Posttransplantation

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Mouse Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1940))

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Abstract

The tracking of the hematopoietic potential of genetically manipulated fluorescent hematopoietic stem cells (HSC) in the bone marrow (BM) allows the assessment of regulatory processes involved in the re-establishment of hematopoiesis posttransplant. Herein, we describe the means to assess the consequence of expressing specific genes in HSC on their engraftment potential posttransplant.

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References

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Authors and Affiliations

  1. Biomedical Manufacturing, CSIRO Clayton, Clayton, VIC, Australia

    Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams & Susan K. Nilsson

  2. Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia

    Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams & Susan K. Nilsson

Authors
  1. Benjamin Cao
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  2. Songhui Li
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  3. Claire Pritchard
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  4. Brenda Williams
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  5. Susan K. Nilsson
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Corresponding author

Correspondence to Susan K. Nilsson .

Editor information

Editors and Affiliations

  1. Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia

    Ivan Bertoncello

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Cao, B., Li, S., Pritchard, C., Williams, B., Nilsson, S.K. (2019). Mouse Hematopoietic Stem Cell Modification and Labelling by Transduction and Tracking Posttransplantation. In: Bertoncello, I. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 1940. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9086-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9086-3_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9085-6

  • Online ISBN: 978-1-4939-9086-3

  • eBook Packages: Springer Protocols

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