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Single-Molecule FRET Methods to Study Glutamate Receptors

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Glutamate Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1941))

Abstract

Single-molecule fluorescence energy transfer methods allow us to determine the complete structural landscape between the donor and acceptor fluorophores introduced on the protein of interest. This method is particularly attractive to study ion channel proteins as single-molecule current recordings have been used to study the function of these proteins for several decades. Here we describe the smFRET method used to study glutamate receptors.

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Acknowledgments

This project was supported by NIH grants R35GM122528 (VJ) and F31GM130035 (RJD) and by the Houston Area Molecular Biophysics Training Program NIH- 2T32 GM008280-26 (DBL).

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Authors and Affiliations

  1. Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX, USA

    Douglas B. Litwin, Ryan J. Durham & Vasanthi Jayaraman

Authors
  1. Douglas B. Litwin
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  2. Ryan J. Durham
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  3. Vasanthi Jayaraman
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Corresponding author

Correspondence to Vasanthi Jayaraman .

Editor information

Editors and Affiliations

  1. Department of Neurology, University of Wisconsin, Madison, WI, USA

    Corinna Burger

  2. ANSER, Homeland Security Studies and Analysis Institute, Falls Church, VA, USA

    Margaret Jo Velardo

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Litwin, D.B., Durham, R.J., Jayaraman, V. (2019). Single-Molecule FRET Methods to Study Glutamate Receptors. In: Burger, C., Velardo, M. (eds) Glutamate Receptors. Methods in Molecular Biology, vol 1941. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9077-1_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9077-1_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9076-4

  • Online ISBN: 978-1-4939-9077-1

  • eBook Packages: Springer Protocols

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