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Oligodendrocyte–Neuron Myelinating Coculture

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Book cover Oligodendrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1936))

Abstract

The preparation of oligodendrocytes and neurons independently in vitro has provided substantial insight into the biology of the process of myelin sheath formation. This chapter describes a myelination system of dorsal root ganglion neurons by independent isolation of oligodendrocyte progenitor cells from either rat or mouse cortex. This in vitro assay can be used to examine the molecular determinants of myelin sheath formation.

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Author information

Authors and Affiliations

  1. MRC Centre for Regenerative Medicine, The Multiple Sclerosis Research Centre, The University of Edinburgh, Edinburgh, UK

    Matthew Swire & Charles ffrench-Constant

Authors
  1. Matthew Swire
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  2. Charles ffrench-Constant
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Corresponding author

Correspondence to Matthew Swire .

Editor information

Editors and Affiliations

  1. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    David A. Lyons

  2. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    Linde Kegel

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Swire, M., ffrench-Constant, C. (2019). Oligodendrocyte–Neuron Myelinating Coculture. In: Lyons, D., Kegel, L. (eds) Oligodendrocytes. Methods in Molecular Biology, vol 1936. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9072-6_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9072-6_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9070-2

  • Online ISBN: 978-1-4939-9072-6

  • eBook Packages: Springer Protocols

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