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Oligodendrocytes pp 1–21Cite as

Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1936))

Abstract

Single-cell RNA sequencing has emerged as a powerful technique for the identification of distinct cell states/populations in complex tissues. We have recently used this technology to investigate heterogeneity of cells of the oligodendrocyte lineage in the mouse central nervous system. In this chapter, we describe methods to perform single-cell RNA sequencing on this glial cell lineage, and discuss experimental and computational approaches to explore the potential and to tackle hurdles associated with this technology.

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Acknowledgments

We would like to thank Elisa Floriddia for proofreading and Amit Zeisel for comments. Work in G.C.-B.’s research group was supported by Swedish Research Council, European Union (FP7/Marie Curie Integration Grant EPIOPC, Horizon 2020 European Research Council Consolidator Grant EPIScOPE), European Committee for Treatment and Research in Multiple Sclerosis, Swedish Brain Foundation, Swedish Cancer Society, Ming Wai Lau Centre for Reparative Medicine, Petrus och Augusta Hedlunds Foundation and Karolinska Institutet.

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Authors and Affiliations

  1. Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden

    Sueli Marques, David van Bruggen & Gonçalo Castelo-Branco

  2. Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, Stockholm, Sweden

    Gonçalo Castelo-Branco

Authors
  1. Sueli Marques
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  2. David van Bruggen
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  3. Gonçalo Castelo-Branco
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Corresponding author

Correspondence to Gonçalo Castelo-Branco .

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Editors and Affiliations

  1. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    David A. Lyons

  2. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    Linde Kegel

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Marques, S., van Bruggen, D., Castelo-Branco, G. (2019). Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System. In: Lyons, D., Kegel, L. (eds) Oligodendrocytes. Methods in Molecular Biology, vol 1936. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9072-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9072-6_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9070-2

  • Online ISBN: 978-1-4939-9072-6

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