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Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods

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Post-Translational Modification of Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1934))

Abstract

Phosphorylation, the process by which a phosphate group is attached to a preexisting protein, is an evolutionarily and metabolically cheap way to change the protein’s surface and properties. It is presumably for that reason that it is the most widespread protein modification: An estimated 10–30% of all proteins are subject to phosphorylation.

MS-based methods are the methods of choice for the identification of phosphorylation sites; however biochemical pre-fractionation and enrichment protocols will be needed to produce suitable samples in the case of low-stoichiometry phosphorylation. Using emerging MS-based technology, the elucidation of the “phosphoproteome,” a comprehensive inventory of phosphorylation sites, will become a realistic goal. However, validating these findings in a cellular context and defining their biological meaning remains a daunting task, which will inevitably require extensive and time-consuming additional biological research.

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Authors and Affiliations

  1. Institute of Clinical Chemistry, University Medical Center Göttingen, Göttingen, Deutschland

    Christof Lenz

  2. Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry, Göttingen, Deutschland

    Christof Lenz

Authors
  1. Christof Lenz
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Correspondence to Christof Lenz .

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Editors and Affiliations

  1. Recombinant Research and Development, Octapharma Biopharmaceuticals GmbH, Heidelberg, Germany

    Christoph Kannicht

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Lenz, C. (2019). Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods. In: Kannicht, C. (eds) Post-Translational Modification of Proteins. Methods in Molecular Biology, vol 1934. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9055-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9055-9_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9053-5

  • Online ISBN: 978-1-4939-9055-9

  • eBook Packages: Springer Protocols

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