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Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1921))

Abstract

The presence of Legionella spp. in natural and man-made water systems is a great public health concern and heavily depends on the presence of free-living amoebae. Taking advantage of the development and affordability of next-generation sequencing technology, we present here a method to characterize the whole bacterial community directly from water samples, as well as from isolated free-living amoebae.

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Acknowledgment

This work was supported by the 2015–2020 State-Region Planning Contracts (CPER), European Regional Development Fund (FEDER), intramural funds from the Centre National de la Recherche Scientifique and the University of Poitiers, and Eau de Paris.

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Authors and Affiliations

  1. Laboratoire Ecologie et Biologie des Interactions, Equipes “Microbiologie de l’Eau” et “Ecologie, Evolution, Symbiose”, Université de Poitiers, UMR CNRS 7267, Poitiers, France

    Vincent Delafont, Yoann Perrin, Didier Bouchon & Yann Héchard

  2. Eau de Paris, Direction de la Recherche et du Développement pour la Qualité de l’Eau, R&D Biologie, Ivry sur Seine, France

    Yoann Perrin & Laurent Moulin

Authors
  1. Vincent Delafont
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  2. Yoann Perrin
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  3. Didier Bouchon
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  4. Laurent Moulin
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  5. Yann Héchard
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Corresponding author

Correspondence to Yann Héchard .

Editor information

Editors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  2. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

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Delafont, V., Perrin, Y., Bouchon, D., Moulin, L., Héchard, Y. (2019). Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_26

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  • DOI: https://doi.org/10.1007/978-1-4939-9048-1_26

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9047-4

  • Online ISBN: 978-1-4939-9048-1

  • eBook Packages: Springer Protocols

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