Abstract
Agrobacterium-mediated transformation is a fast and efficient method for genome modification in plants. In this protocol, we apply this technique for the analysis of root microRNA functionality. The induction of hairy roots constitutively overexpressing a given microRNA precursor allows us, in a simple way, to modify the accumulation of specific mature microRNA and analyze the consequence of this alteration on a phenotype of interest. This method generates ready-to-phenotype “composite plants” with untransformed aerial part and microRNA-overexpressing root system, in about 20 days.
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Acknowledgments
The work in our group has been supported by Dirección General de Asuntos del Personal Académico (DGAPA)—UNAM (grants no. PAPIIT IN200816 and IA203218).
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Formey, D., Martín-Rodríguez, J.Á., Hernández, G. (2019). Functional Analysis of Root microRNAs by a Constitutive Overexpression Approach in a Composite Plant System. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_16
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DOI: https://doi.org/10.1007/978-1-4939-9042-9_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9041-2
Online ISBN: 978-1-4939-9042-9
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