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In Situ Localization of Small RNAs in Plants

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Plant MicroRNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1932))

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Abstract

Small RNAs have vital roles in numerous aspects of plant biology. Deciphering their precise contributions requires knowledge of a small RNA’s spatiotemporal pattern of accumulation. The in situ hybridization protocol described here takes advantage of locked nucleic acid (LNA) oligonucleotide probes to visualize small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within 2–6 days.

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Acknowledgments

Damianos Skopelitis was supported by an HFSP long-term postdoctoral fellowship (LT000257/2009). Work on small RNA regulation in the Timmermans lab is supported by grants from the National Science Foundation (IOS-1355018), the Deutsche Forschungsgemeinschaft (SFB 1101 project C06), and an Alexander von Humboldt Professorship.

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Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

    Cristina F. Marco, Damianos S. Skopelitis & Marja C. P. Timmermans

  2. Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany

    Marja C. P. Timmermans

Authors
  1. Cristina F. Marco
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  2. Damianos S. Skopelitis
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  3. Marja C. P. Timmermans
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Corresponding author

Correspondence to Marja C. P. Timmermans .

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Editors and Affiliations

  1. Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Guanajuato, Mexico

    Stefan de Folter

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Marco, C.F., Skopelitis, D.S., Timmermans, M.C.P. (2019). In Situ Localization of Small RNAs in Plants. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9042-9_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9041-2

  • Online ISBN: 978-1-4939-9042-9

  • eBook Packages: Springer Protocols

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