Abstract
Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. In this work, we describe a tissue printing and a dot blot hybridization protocol for miRNA detection and localization in plant tissues, which opens the possibility of analyzing spatiotemporal expression patterns of miRNAs.
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References
Varner JE, Ye Z (1994) Tissue printing. FASEB J 8:378–384
Pont-Lezica RF (2015) Localizing proteins by tissue printing. Methods Mol Biol 1312:93–104
Rosas-Cardenas Fde F, Escobar-Guzman R, Cruz-Hernandez A, Marsch-Martinez N, de Folter S (2015) An efficient method for miRNA detection and localization in crop plants. Front Plant Sci 6:99
Rosas-Cardenas Fde F, Caballero-Perez J, Gutierrez-Ramos X, Marsch-Martinez N, Cruz-Hernandez A, de Folter S (2015) miRNA expression during prickly pear cactus fruit development. Planta 241:435–448
Pallás V, Sánchez-Navarro JA, Kinard GR, Di Serio F (2017) Chapter 35 - molecular hybridization techniques for detecting and studying viroids A2 - Hadidi, Ahmed. In: Flores R, Randles JW, Palukaitis P (eds) Viroids and satellites. Academic Press, Boston, pp 369–379
Simon A, Ruiz L, Velasco L, Janssen D (2018) Absolute quantification of tomato leaf curl new delhi virus spain strain, ToLCNDV-ES: virus accumulation in a host-specific manner. Plant Dis 102:165–171
Liu YH, Offler CE, Ruan YL (2014) A simple, rapid, and reliable protocol to localize hydrogen peroxide in large plant organs by DAB-mediated tissue printing. Front Plant Sci 5:745
Pluskota W, Bradford K, Nonogaki H (2011) Tissue-printing methods for localization of RNA and proteins that control seed dormancy and germination. Methods Mol Biol 773:329–339
Esteves F, Teixeira Santos M, Eiras-Dias JE, Fonseca F (2013) Molecular data mining to improve antibody-based detection of grapevine leafroll-associated virus 1 (GLRaV-1). J Virol Methods 194:258–270
Damm K, Bach S, Müller KM, Klug G, Burenina OY, Kubareva EA, Grünweller A, Hartmann RK (2015) Improved Northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes. Methods Mol Biol 1296:41–51
Pall GS, Hamilton AJ (2008) Improved northern blot method for enhanced detection of small RNA. Nat Protoc 3:1077–1084
Zhang X, Dong H, Tian Y (2015) Conventional miRNA detection strategies. In: MicroRNA detection and pathological functions. Springer, Berlin, pp 23–35
Beckmann BM, Grünweller A, Weber MHW, Hartmann RK (2010) Northern blot detection of endogenous small RNAs (∼14 nt) in bacterial total RNA extracts. Nucleic Acids Res 38:e147
Kim SW, Li Z, Moore PS, Monaghan AP, Chang Y, Nichols M, John B (2010) A sensitive non-radioactive northern blot method to detect small RNAs. Nucleic Acids Res 38:e98
Acknowledgments
We thank the Mexican National Council of Science and Technology (CONACyT) for a fellowship to MMN. This work was financed by the CONACyT grant CB-2013-221522, and SIP grants 20170477 and 20180545. Work in the SDF laboratory was financed by the CONACyT grants CB-2012-177739 and FC-2015-2/1061.
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Martínez Núñez, M., de Folter, S., Rosas-Cárdenas, F.d.F. (2019). Detection of miRNAs by Tissue Printing and Dot Blot Hybridization. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_11
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DOI: https://doi.org/10.1007/978-1-4939-9042-9_11
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