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Development of a Pedigreed Sorghum Mutant Library

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Sorghum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1931))

Abstract

Induced mutagenesis is a powerful approach to generate variations for elucidation of gene function and to create new traits for breeding. Here, we described a procedure to develop a pedigreed mutant library through chemical mutagenesis with ethylmethane sulfonate (EMS) treated seeds in sorghum and discussed its potential to generate new traits for sorghum improvement. Unlike random mutagenesis, a pedigreed mutant library, once properly established, can serve as a powerful resource to isolate and recover mutations of both agronomical and biological importance. With the development of affordable and high-throughput next-generation sequencing technologies, identification of causal mutations from a mutant library with a uniform genetic background becomes increasingly efficient and cost-effective. Fast causal gene discovery from mutant libraries combined with precise genome editing techniques will accelerate incorporation of new traits and revolutionize crop breeding.

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Acknowledgment

We thank the United Sorghum Checkoff for funding and Lan Liu-Gitz for technical support.

Disclaimer: Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Plant Stress and Germplasm Development Unit, USDA-ARS, Lubbock, TX, USA

    Junping Chen, Guihua Zou & Zhanguo Xin

  2. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang Province, China

    Guihua Zou

Authors
  1. Junping Chen
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  2. Guihua Zou
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  3. Zhanguo Xin
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Corresponding author

Correspondence to Zhanguo Xin .

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Editors and Affiliations

  1. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Zuo-Yu Zhao

  2. UC-ANR Kearney Agricultural Research and Extension (KARE) Center, Parlier, CA, USA

    Jeff Dahlberg

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Chen, J., Zou, G., Xin, Z. (2019). Development of a Pedigreed Sorghum Mutant Library. In: Zhao, ZY., Dahlberg, J. (eds) Sorghum. Methods in Molecular Biology, vol 1931. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9039-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9039-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9038-2

  • Online ISBN: 978-1-4939-9039-9

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