Abstract
The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.
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Acknowledgments
This work was supported by the Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant and the Singapore Ministry of Education (MOE) under its MOE Academic Research Fund (AcRF) Tier 1 (2014-T1-001-141) and MOE-AcRF Tier 2 (MOE2017-T2-2-004) grants to N.K.V.
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Prasannan, P., Verma, N.K. (2019). Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis. In: Verma, N. (eds) T-Cell Motility. Methods in Molecular Biology, vol 1930. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9036-8_7
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DOI: https://doi.org/10.1007/978-1-4939-9036-8_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9035-1
Online ISBN: 978-1-4939-9036-8
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