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Assessing the Quality of Recombinant Products Made in Yeast

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Recombinant Protein Production in Yeast

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1923))

Abstract

The product quality of recombinant proteins is of major importance for their intended purpose. The initial characterization of both simple and complex products should be performed as soon as practical. However, to comply with this high standard, appropriate selection of complementary methods is required. Therefore, conventional and sophisticated techniques are available, providing diverse information about the product quality.

In this chapter methods are presented, which enable the determination of the overall protein quality, their aggregation and peptide composition. Methods applied for the determination of posttranslational modifications such as glycan analysis are not described. In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, University of Natural Resources and Life Sciences Vienna (BOKU), Vienna, Austria

    Karola Vorauer-Uhl & Gabriele Lhota

Authors
  1. Karola Vorauer-Uhl
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  2. Gabriele Lhota
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Corresponding author

Correspondence to Karola Vorauer-Uhl .

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Editors and Affiliations

  1. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Brigitte Gasser

  2. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Diethard Mattanovich

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Vorauer-Uhl, K., Lhota, G. (2019). Assessing the Quality of Recombinant Products Made in Yeast. In: Gasser, B., Mattanovich, D. (eds) Recombinant Protein Production in Yeast. Methods in Molecular Biology, vol 1923. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9024-5_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9024-5_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9023-8

  • Online ISBN: 978-1-4939-9024-5

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