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Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells

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Kidney Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1926))

Abstract

Nephrons, the functional units of the kidney, are derived from nephron progenitor cells (NPCs). Here, we describe methods to reconstruct nephron tissue via induction of NPCs from mouse and human pluripotent stem cells, which mimic multistep developmental signals in vivo. Induced NPCs differentiate into three-dimensional nephron structures, including glomerular podocytes and nephric tubules, which are useful for studying early stages of kidney specification and morphogenetic processes in the context of normal development or disease.

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Authors and Affiliations

  1. Department of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan

    Yasuhiro Yoshimura & Ryuichi Nishinakamura

  2. Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin, Germany

    Atsuhiro Taguchi

Authors
  1. Yasuhiro Yoshimura
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  2. Atsuhiro Taguchi
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  3. Ryuichi Nishinakamura
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Corresponding author

Correspondence to Ryuichi Nishinakamura .

Editor information

Editors and Affiliations

  1. Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland

    Seppo Vainio

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Yoshimura, Y., Taguchi, A., Nishinakamura, R. (2019). Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells. In: Vainio, S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9021-4_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9020-7

  • Online ISBN: 978-1-4939-9021-4

  • eBook Packages: Springer Protocols

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