Abstract
Optical projection tomography (OPT) is a 3D imaging technology. The 3D tomographic reconstruction permits precise analysis and quantification of various structures in developing embryonic tissues and adult organs of small rodents or biopsies. OPT enables detailed and accurate studies of kidney organogenesis, namely, ureteric tree branching morphogenesis and nephron quantification.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Sharpe J, Ahlgren U, Perry P et al (2002) Optical projection tomography as a tool for 3D microscopy and gene expression studies. Science 296:541–545
Wilkinson D (1998) In situ hybridization: a practical approach (practical approach series). Oxford University Press, Oxford
Susaki E, Tainaka K, Perrin D et al (2014) Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. Cell 157:726–739
Short K, Hodson M, Smyth I (2010) Tomographic quantification of branching morphogenesis and renal development. Kidney Int 77:1132–1139
Watson T, Andrews N, Davis S et al (2017) OPTiM: Optical projection tomography integrated microscope using open-source hardware and software. PLoS One 12:e0180309
Wong M, Dazai J, Walls J et al (2013) Design and implementation of a custom built optical projection tomography system. PLoS One 8:e73491
Gualda E, Vale T, Almada P et al (2013) OpenSpinMicroscopy: an open-source integrated microscopy platform. Nat Methods 10:599–600
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Prunskaite-Hyyryläinen, R. (2019). Optical Projection Tomography Imaging to Study Kidney Organogenesis. In: Vainio, S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_16
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9021-4_16
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9020-7
Online ISBN: 978-1-4939-9021-4
eBook Packages: Springer Protocols