Skip to main content
SpringerLink
Log in
Book cover

Kidney Organogenesis pp 185–199Cite as

Optical Projection Tomography Imaging to Study Kidney Organogenesis

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1926))

Abstract

Optical projection tomography (OPT) is a 3D imaging technology. The 3D tomographic reconstruction permits precise analysis and quantification of various structures in developing embryonic tissues and adult organs of small rodents or biopsies. OPT enables detailed and accurate studies of kidney organogenesis, namely, ureteric tree branching morphogenesis and nephron quantification.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Sharpe J, Ahlgren U, Perry P et al (2002) Optical projection tomography as a tool for 3D microscopy and gene expression studies. Science 296:541–545

    Article  CAS  Google Scholar 

  2. Wilkinson D (1998) In situ hybridization: a practical approach (practical approach series). Oxford University Press, Oxford

    Google Scholar 

  3. Susaki E, Tainaka K, Perrin D et al (2014) Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. Cell 157:726–739

    Article  CAS  Google Scholar 

  4. Short K, Hodson M, Smyth I (2010) Tomographic quantification of branching morphogenesis and renal development. Kidney Int 77:1132–1139

    Article  Google Scholar 

  5. Watson T, Andrews N, Davis S et al (2017) OPTiM: Optical projection tomography integrated microscope using open-source hardware and software. PLoS One 12:e0180309

    Article  Google Scholar 

  6. Wong M, Dazai J, Walls J et al (2013) Design and implementation of a custom built optical projection tomography system. PLoS One 8:e73491

    Article  CAS  Google Scholar 

  7. Gualda E, Vale T, Almada P et al (2013) OpenSpinMicroscopy: an open-source integrated microscopy platform. Nat Methods 10:599–600

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland

    Renata Prunskaite-Hyyryläinen

Authors
  1. Renata Prunskaite-Hyyryläinen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Renata Prunskaite-Hyyryläinen .

Editor information

Editors and Affiliations

  1. Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland

    Seppo Vainio

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Prunskaite-Hyyryläinen, R. (2019). Optical Projection Tomography Imaging to Study Kidney Organogenesis. In: Vainio, S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-9021-4_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9020-7

  • Online ISBN: 978-1-4939-9021-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation