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Kidney Organogenesis pp 143–149Cite as

Virus as Renal Functional Genetic Tools

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1926))

Abstract

Viral vectors enable efficient transfection of ectopic DNA into hard to transfect cells. Viral vectors are normally used to obtain permanent modification of target cells, and tissues expect for the cases where integrase-deficient viruses are used. Here we describe a method to stably transfect metanephric mesenchyme cells isolated from the murine embryonic kidney at day E11.5. Using this method, it is possible to transfect hard to transfect cells and successfully evade host tissue immune response. Due to these advantages, this method has become one of the most frequently used in generating stable cell line, manipulation of tissues, and gene therapy.

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Authors and Affiliations

  1. Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu, Oulu, Finland

    Abhishek Sharma

  2. Faculty of Veterinary Medicine, Section of Pathology, Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland

    Sandhanakrishnan Cattavarayane

Authors
  1. Abhishek Sharma
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  2. Sandhanakrishnan Cattavarayane
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Corresponding author

Correspondence to Abhishek Sharma .

Editor information

Editors and Affiliations

  1. Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland

    Seppo Vainio

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Sharma, A., Cattavarayane, S. (2019). Virus as Renal Functional Genetic Tools. In: Vainio, S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9021-4_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9020-7

  • Online ISBN: 978-1-4939-9021-4

  • eBook Packages: Springer Protocols

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