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MCU Regulation in Lipid Bilayer and Electrophysiological Recording

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Calcium Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1925))

Abstract

The mitochondrial calcium uniporter (MCU) and the mitochondrial calcium uniporter dominant negative b- subunit (MCUb) are pore-forming components of the uniporter complex. We expressed these MCU subunits in cell-free transcription/translation systems, and we studied them, at the single molecule level, using the electrophysiological technique of planar lipid bilayer.

We showed that MCU gives rise to single-channel Ca2+ currents. In contrast, MCUb alone does not display calcium-permeable channel activity, while the co-expression of MCUb:MCU drastically alters the calcium permeation mediated by MCU subunit.

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Author information

Authors and Affiliations

  1. Department of Biology, University of Padua, Padua, Italy

    Vanessa Checchetto & Ildikò Szabò

Authors
  1. Vanessa Checchetto
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  2. Ildikò Szabò
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Corresponding author

Correspondence to Vanessa Checchetto .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Anna Raffaello

  2. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Denis Vecellio Reane

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Checchetto, V., Szabò, I. (2019). MCU Regulation in Lipid Bilayer and Electrophysiological Recording. In: Raffaello, A., Vecellio Reane, D. (eds) Calcium Signalling. Methods in Molecular Biology, vol 1925. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9018-4_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9018-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9017-7

  • Online ISBN: 978-1-4939-9018-4

  • eBook Packages: Springer Protocols

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