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Grafting as a Tool for Study of Phototropism in Phycomyces

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Phototropism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1924))

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Abstract

Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.

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References

  1. Cerdá-Olmedo E, Lipson ED (1987) A biography of Phycomyces. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New York

    Google Scholar 

  2. Eslava AP (1987) Genetics. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New York

    Google Scholar 

  3. Suárez T, Orejas M, Eslava AP (1985) Isolation, regeneration, and fusion of Phycomyces blakesleeanus spheroplasts. Exp Mycol 9:203–211

    Article  Google Scholar 

  4. Suárez T, Orejas M, Arnau J, Torres-Martínez S (1987) Protoplast formation and fusion. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New York

    Google Scholar 

  5. Fukui J, Choi KS, Miyazaki A, Ootaki T, Oikawa T (2001) Analysis of the formation of protoplasts and regeneration of cells in Phycomyces blakesleeanus. J Microbiol Biotechnol 11:72–78

    CAS  Google Scholar 

  6. Heisenberg M, Cerdá-Olmedo E (1968) Segregation of heterokaryons in the asexual cycle of Phycomyces. Mol Gen Genet 102:187–195

    Article  CAS  Google Scholar 

  7. Ootaki T (1987) Heterokaryon formation. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New York

    Google Scholar 

  8. Ootaki T, Miyazaki A, Choi KS (2002) Positional differentiation for development along the sporangiophore in Phycomyces blakesleeanus. Mycoscience 43:239–245

    Article  Google Scholar 

  9. Ootaki T (1973) A new method for heterokaryon formation in Phycomyces. Mol Gen Genet 121:49–56

    Article  CAS  Google Scholar 

  10. Gruen HE, Ootaki T (1970) Regeneration on Phycomyces sporangiophores. I. Induction by decapitation and basal cutting on sporangiophores attached to mycelium and after isolation. Can J Bot 48:55–65

    Article  Google Scholar 

  11. Ootaki T, Gruen HE (1970) Regeneration on Phycomyces sporangiophores. II. Regeneration on isolated segments from different positions along the sporangiophore axis. Can J Bot 48:95–109

    Article  Google Scholar 

  12. Gruen HE, Ootaki T (1972) Regeneration on Phycomyces sporangiophores. III. Grafting of sporangiophore segments, weight changes, and protoplasmic streaming in relation to regeneration. Can J Bot 50:139–158

    Article  Google Scholar 

  13. Ootaki T, Fischer EP, Lockhart P (1974) Complementation between mutants of Phycomyces with abnormal phototropism. Mol Gen Genet 131:233–246

    Article  Google Scholar 

  14. Ootaki T, Kinno T, Yoshida K, Eslave AP (1977) Complementation between Phycomyces mutants of mating type (+) with abnormal phototropism. Mol Gen Genet 152:245–251

    Article  Google Scholar 

  15. Alvarez MI, Ootaki T, Eslava AP (1983) Mutants of Phycomyces with abnormal phototropism induced by ICR-170. Mol Gen Genet 191:507–511

    Article  CAS  Google Scholar 

  16. Ootaki T, Lighty AC, Delbrück M (1973) Complementation between mutants of Phycomyces deficient with respect to carotenogenesis. Mol Gen Genet 121:57–70

    Article  CAS  Google Scholar 

  17. Koga K, Ootaki T (1983) Complementation analysis among piloboloid mutants of Phycomyces blakesleeanus. Exp Mycol 7:161–169

    Article  Google Scholar 

  18. Delbrück M, Ootaki T (1979) An unstable nuclear gene in Phycomyces. Genetics 92:27–48

    PubMed  PubMed Central  Google Scholar 

  19. Fukui J, Miyazaki A, Ootaki T (2000) Isolation and characterization of chlorate resistance mutants from nitrate-nonutilizing fungus Phycomyces blakesleeanus. Mycoscience 41:633–640

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, Faculty of Science and Engineering, Ishinomaki Senshu University, Ishinomaki, Miyagi, Japan

    Atsushi Miyazaki

  2. Tohoku University, Sendai, Japan

    Tamotsu Ootaki

Authors
  1. Atsushi Miyazaki
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  2. Tamotsu Ootaki
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Corresponding author

Correspondence to Atsushi Miyazaki .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, Japan

    Kotaro T. Yamamoto

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Miyazaki, A., Ootaki, T. (2019). Grafting as a Tool for Study of Phototropism in Phycomyces. In: Yamamoto, K. (eds) Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9015-3_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9015-3_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9014-6

  • Online ISBN: 978-1-4939-9015-3

  • eBook Packages: Springer Protocols

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